- Stable Cell Line Development
Stable cell lines express exogenous genes constitutively. Due to their high stability, they are suitable for recombinant protein and antibody production to facilitate applications, such as structural biology research, structure-function studies, and the manufacture of biologics. To support clients’ research, Sino Biological provides a one-stop service package from gene synthesis to stable cell line generation. Dedicated to recombinant protein expression, our experts have gained extensive experience in optimizing the generation of high-production stable cell lines.
Service Details for Stable Cell Line Development
1-2 Weeks
2-3 Weeks
2 Weeks
10-24 Weeks
2-3 Weeks
Gene synthesis and codon optimization (Optional)
Vector construction
Transient expression evaluation
Stable cell line development
(Optional)
Large-scale expression and purification
(Optional)
Simply the protein/gene sequence or the plasmid.
- Cloning cDNA into expression vectors
- Plasmid sequencing
- Plasmid preparation
- Transient expression
- Expression level detection by ELISA or WB
- Stable transfection and screening
- High expression clone screening and stability test
- Cell banking
- QC analysis
Deliverables
Deliverables
Deliverables
Deliverables
- Sequencing report (if requested)
- Evaluation report
- Expression report
- 2-3 stable clones (optional)
- Purified proteins
- CoA