Exonuclease III is a 3’→5’ exonuclease which acts by digesting one strand of a dsDNA duplex at a time or digesting the RNA strand of an RNA-DNA heteroduplex. Exonuclease III breaks phosphodiester bonds on the 5’ side of AP sites in both dsDNA and ssDNA and removes 3’ terminal groups on dsDNA, efficiently degrades 3’ recessed but not 3’ protruding DNA ends (creating 5’ overhangs)

Concentration	Size	Price (US)
100,000 units/mL	100,000 units	US$340
100,000 units/mL	50,000 units	US$190
100,000 units/mL	20,000 units	US$84

Purified from a strain of E. coli that expresses the recombinant Exonuclease III gene

Supplied in

10 mM Tris-HCl
100 mM NaCl
0.1 mM EDTA
1 mM DTT
50% glycerol
pH 7.4 @ 25°C

One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble total nucleotide in 30 minutes at 37°C

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