One-Stop Solution For CceAAV Production Service
A conventional recombinant single-stranded AAV (ssAAV) and self-complementary AAV (scAAV) package the genome with wild-type ITRs or mutated ITRs flanking an expression cassette encoding a gene of interest. Upon uncoating in target cells, scAAV can rapidly form an intra-molecular double-stranded DNA, facilitating high-level transgene expression.
In our proprietary cceAAV (covalently closed end double stranded AAV) technology, we used a 56-bp recognition sequence of protelomerase (Tel N) to covalently join the top and bottom strands, allowing the vector to be generated with just a single ITR. To produce cceAAV vectors, the vector plasmid is initially digested with Tel N, purified, and then subjected to a standard triple-plasmid transfection protocol followed by traditional AAV vector purification procedures.
CceAAV allows for an increased packaging capacity compared to scAAV vectors due to a higher formation rate of hairpin loops in vitro and fewer nucleotides than the mutated ITR. Analysis on Alkaline Agarose gel indicates that cceAAV viruses have a lower fraction of unexpected partial genomes compared to scAAV, suggesting potential enhancement in potency and reduced safety concerns.
We provide one-stop solution for cceAAV Production Service including plasmid preparation, high-quality at affordably priced Tel N enzyme and cceAAV production service to meet the customer convenience of cceAAV quick testing.
One-Stop Solution For CceAAV Production Service
| category | ID | Sample name | Specification | Description | Add to cart |
|---|---|---|---|---|---|
| Enzyme | Tel N enzyme | 500U/1000U/2500U/5000U | Cuts dsDNA at a TelN recognition sequence (56 bp) and leaves covalently closed ends at the site of cleavage | ||
| Plasmid preparation | pCceAAV-CB-EGFP | 5mg/10mg | pAAV with one Tel N and CB promoter and EGFP expression gene (without Tel N enzyme treatment) | ||
| Plasmid preparation | pScAAV-CB-EGFP | 5mg/10mg | pAAV with one trs deleted ITR and CB promoter and EGFP expression gene | ||
| Plasmid preparation | pCceAAV-CB-EGFP (Tel N) | 5mg/10mg | pAAV with one Tel N and CB promoter and EGFP expression gene treated with Tel N and purified, could be used for AAV production | ||
| Plasmid preparation | pCceAAV-CB-Gluc | 5mg/10mg | pAAV with one Tel N and CB promoter and Gluc expression gene (without Tel N enzyme treatment) | ||
| Plasmid preparation | pScAAV-CB-Gluc | 5mg/10mg | pAAV with one trs deleted ITR and CB promoter and Gluc expression gene | ||
| Plasmid preparation | pCceAAV-CB-Gluc (Tel N) | 5mg/10mg | pAAV with one Tel N and CB promoter and Gluc expression gene treated with Tel N and purified, could be used for AAV production | ||
| Plasmid preparation | pCceAAV-TTR-hFIX | 5mg/10mg | pAAV with one Tel N and murine promoter TTR and R338LhFIXco expression gene (without Tel N enzyme treatment) | ||
| Plasmid preparation | pScAAV-TTR-hFLX | 5mg/10mg | pAAV with one trs deleted ITR and murine promoter TTR and R338LhFIXco expression gene | ||
| Plasmid preparation | pCce-TTR-hFIX (Tel N) | 5mg/10mg | pAAV with one Tel N and murine promoter TTR and R338LhFIXco expression gene treated with Tel N and purified, could be used for AAV production | ||
| AAV production | scAAV-CB-EGFP | scAAV package with CB promoter and GFP expression gene as required serotype | |||
| AAV production | cceAAV- CB-EGFP | cceAAV package with CB promoter and GFP expression gene as required serotype | |||
| AAV production | scAAV- CB-Gluc | cceAAV package with CB promoter and GFP expression gene as required serotype | |||
| AAV production | cceAAV- CB-Gluc | cceAAV package with CB promoter and Gluc expression gene as required serotype | |||
| AAV production | ScAAV-TTR-hFLX | cceAAV package with murine promoter TTR and R338LhFIXco expression gene as required serotype | |||
| AAV production | cceAAV-TTR-hFIX | cceAAV package with murine promoter TTR and R338LhFIXco expression gene as required serotype |