pHAE-2
AAV packaging plasmid expressing AAV2 Rep protein and HAE-2 capsid protein
Using a plasmid library of chimeric AAV capsids we performed directed evolution of AAV on HAE. After five successive rounds of screenings,
two novel AAV variants were identified with greater transduction efficiency for HAE than the parental serotypes AAV-1 and AAV-6.
Li, W., et al., Generation of Novel AAV Variants by Directed Evolution for Improved CFTR Delivery to Human Ciliated Airway Epithelium. Molecular Therapy, 2009. 17(12): p. 2067-2077.
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