pAAV-PT1
AAV packaging plasmid expressing AAV2 Rep protein and AAV-PT1 capsid protein
AAV-PT1 is a variation(TEN to EKH at 376-378 in VR-III, S518K in VR-VI, and ND to SE at 694-695, Q697T and T706A in VR-IX) of AAV5, which is a novel AAV capsid capable of efficiently transducing the retina after intravitreal injection.
Cai, Z., et al. Novel Intravitreal Retina-Targeting and Immune-Evading Adeno-Associated Virus Capsid Variant. Preprints, 2024. 202409.2179.
>pAAV-PT1
MAADGYLPDWLEDNLSEGIREWWALKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLQAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPTGKRIDDHFPKRKKARTEEDSKPSTSSDAEAGPSGSQQLQIPAQPASSLGADTMSAGGGGPLGDNNQGADGVGNASGDWHCDSTWMGDRVVTKSTRTWVLPSYNNHQYREIKSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLINNYWGFRPRSLRVKIFNIQVKEVTVQDSTTTIANNLTSTVQVFTDDDYQLPYVVGNGTEGCLPAFPPQVFTLPQYGYATLNRDNEKHPTERSSFFCLEYFPSKMLRTGNNFEFTYNFEEVPFHSSFAPSQNLFKLANPLVDQYLYRFVSTNATGGVQFNKNLAGRYANTYKNWFPGPMGRTQGWNLGSGVNRASVSAFATTNRMELEGASYQVPPQPNGMTNNLQGKNTYALENTMIFNSQPANPGTTATYLEGNMLITSESETQPVNRVAYNVGGQMATNAQNASTAPTAGTYNLQEIVPGSVWMERDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPGNITSFSDVPVSSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYSEPTFVDFAPDSAGEYRTTRPIGTRYLTRPL
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