- Antibody Humanization Development
Non-humanized antibodies can induce severe immunological reactions. For instance, the use of mouse monoclonal antibodies in clinical settings is hindered by the human anti-mouse antibody (HAMA) response. Humanizing these antibodies, whether mouse monoclonal antibodies or alpaca single-domain antibodies enhances their resemblance to naturally occurring human antibodies, thus significantly improving their in vivo tolerability.
Nikebio offers top-notch monoclonal antibody humanization services utilizing complementarity-determining region (CDR) grafting technology and computer-aided molecular modeling.
Service Details for Single B Cell Antibody Development:
| Milestone | Items | Detailed information | Time (Week) | Deliverable |
|---|---|---|---|---|
| 1 | Affinity validation | Antibody/antigen affinity validation | 1 Weeks | • Top three humanized antibodies, 0.2~0.5 mg per strain (at least 1 humanized ab • Antibody sequences • Plasmids containing antibody sequences • Remaining antigens • CoA |
| 2 | Preparation of chimeric antibody | Transient expression and purification Chimeric antibody-antigen affinity validation | 2-3 Weeks | |
| 3 | Preparation of humanized variants | 3D structural modeling of antibodies Back mutation Vector construction and plasmid preparation Transient expression and purification Affinity detection: ELISA, Biacore/Octet | 1 Weeks |