- Baculovirus-insect Expression Service
Nikebio offers recombinant protein production services in baculovirus cell expression system. With the help of our optimized insect expression system, our team has rich experience in tailoring various challenging protein targets, including but not limited to toxic proteins, kinases.
Advantages of the baculovirus-insect system:
• Cost-effective
• Easily scalable
• Various post-translational modifications available
• Useful for large protein targets
• Suitable for challenging targets, such as kinases and toxic proteins
Service Details for Baculovirus-insect Expression
1-2 Weeks
1-2 Weeks
2-3 Weeks
2-3 Weeks
2-3 Weeks
Gene synthesis and codon optimization (Optional)
Vector construction
Virus packaging and amplification
Protein expression and purification
Large-scale expression and purification
(Optional)
Simply the protein/gene sequence or the plasmid.
- Cloning cDNA into expression vectors
- Plasmid sequencing
- Plasmid preparation
- Virus packaging
Virus amplification
- Cell infection
- Pilot expression and purification trial
QC analysis
- 10-100+ mg
- QC analysis
Deliverables
Deliverables
Deliverables
- Sequencing report (if requested)
- 0.1-0.5 mg purified proteins (if feasible)
- CoA
- Purified proteins
- CoA