- Enzyme development
Enzymes are proteins that accelerate chemical reactions and are crucial for various physiological processes. We offers recombinant enzyme production services and customized assay development for a range of enzymes. We work closely with your team to tailor process, ensuring they meet your specific requirements and timelines.
Our services include:
- Proteins production with high biological activity, available in full-length or truncated forms, with untagged or tagged versions
- Create optimized assay protocol/conditions for the target protein
- Comprehensive assay validation through comparison with established inhibitory/stimulatory compounds
- Determination of kinetic parameters for the target protein
Service Details for Enzyme Development
Cloning
Expression
Purification &Modification
Characterization
Primers are designed for gene amplification from human cDNA libraries, and cloned into vectors to achieve optimal expression
An expression system is selected to best fit the client’s requirements, considering the required post translational modifications and specific enzymatic activity needs.
Target protein purification follows standard protocols, with options for phosphorylation, tag removal, or buffer exchange as directed by the client.
The final protein product is tested to ensure it meets the client-specified standards for concentration, purity and activity.