- Recombinant Protein Production Services
Recombinant proteins is a technology, which involves inserting genes responsible for encoding the targeted proteins into expression platforms like E.coli, mammalian cells, or insect cells. This method facilitates the extensive production of proteins with precise structures and functions, serving as a cornerstone in diverse fields such as pharmaceutical development, diagnostic testing, vaccine and fundamental biological research.
Nikebio offers one-stop services for recombinant protein production with our advanced protein expression platform. Our full services include gene synthesis, protein codon optimization, protein expression vector design, large scale cell culture and fermentation, protein purification and quality control test.
Recombinant Protein Production Technology
The production of recombinant proteins spans the complete process from gene cloning to protein purification, with the objective of yielding proteins of superior quality for subsequent use in research, diagnostics, and therapeutic treatments. Achieving optimal production levels necessitates meticulous attention to factors such as promoter efficacy, codon optimization and host strain. After expression, the proteins are collected and undergo a series of downstream processes, including cell disruption, purification (typically through affinity chromatography), and quality control to confirm their activity.
Recombinant Protein Expression
To meet the demands of applications in terms of yield, scalability, and post-translational modifications, a range of expression systems have been developed, including prokaryotic systems and eukaryotic systems (insect, and mammalian cells). By appropriately selecting host cells, expression vectors, and culture conditions, it is possible to regulate expression levels, ensure proper protein folding and maintain biological activity.
Recombinant Protein Purification
The primary objective is to efficiently isolate the target protein from host cell lysates or culture supernatants while preserving its conformation and biological activity. Commonly employed purification techniques include affinity chromatography, ion-exchange chromatography, and size-exclusion chromatography, which enable a stepwise increase in purity while maintaining its activity.