- Mammalian Transient Expression Service
Nikebio is a leading expert in preparing proteins of all types using our featured mammalian transient expression system. We specialize in HEK293 and CHO cells. We also provide flexible add-on services to meet custom requirements, including tag removal, endotoxin removal, SEC-HPLC analysis.
Our proprietary high-density cell culture technology, combined with proprietary transfection reagents, medium formulations, and high-efficiency expression vectors, maximizes cell longevity, increases the expression success rate, and reduces customer costs. Our one-stop service offers high-yield, high-expression proteins with post-translational modifications and correct folding.
Service Details for Mammalian Transient Expression
1-2 Weeks
1-2 Weeks
2-3 Weeks
3-4 Weeks
Gene synthesis and codon optimization (Optional)
Vector construction
Protein expression and purification
Large-scale expression and purification
(Optional)
Simply provide the protein/gene sequence or the plasmid.
- Cloning cDNA into expression vectors
- Plasmid sequencing
- Plasmid preparation
- Transient transfection of HEK293/CHO cells
- Protein purification
- QC analysis (SDS-PAGE, UV, etc.)
- 10-100+ mg
- QC analysis
Deliverables
Deliverables
Deliverables
- Sequencing report (if requested)
- 0.1-0.5 mg purified proteins (if feasible)
- CoA
- Purified proteins
- CoA