- Bacterial Protein Expression Service
Nikebio provides recombinant protein expression in E.coli with efficient and cost-effective production. E.coli is a popular host because of its high yield, low cost and convenience of manipulation. The E.coli protein expression system act as a principal tool for recombinant protein expression and biotechnological industrial development, especially for small protein production.
E.coli serves as an attractive host system due to its well-characterized genetic background, high yield, robust production and ease of scaling. Nikebio offers one-stop services for high-quality E.coli protein expression, with abundant experience in insoluble protein, soluble protein expression and protein refolding.
Service Details for Bacterial Protein Expression
1-2 Weeks
1-2 Weeks
1-2 Weeks
2-3 Weeks
2-3 Weeks
Gene synthesis and codon optimization (Optional)
Vector construction
Protein expression testing
Scalable expression and 1-2 step purification
Large-scale expression and purification
(Optional)
Simply provide the protein/gene sequence or the plasmid.
- Cloning cDNA into expression vectors
- Plasmid sequencingl
- Plasmid preparationl
lE.coli strain transformation
lPilot expression and purification trial
lProtein expression evaluation (SDS-PAGE)
lSoluble or insoluble protein purification
lProtein refolding and purification (inclusion bodies)
QC analysis
- 10-100+ mg
- QC analysis
Deliverables
Deliverables
Deliverables
Deliverables
Sequencing report (if requested)
- lExpression evaluation result
l0.5-1 mg purified proteins (if feasible)
- CoA
- Purified proteins
- CoA