Phi29 DNA Polymerase
Overview
Phi29 DNA Polymerase responsible for the replication of the Bacillus Subtilis phage φ29. The enzyme is a highly processive DNA polymerase (up to 70kb insertions) with exceptional strand displacement properties and a 3’→5’ proofreading exonuclease function.
| Catalog # | Concentration | Size | Price (US) |
|---|---|---|---|
| 10,000 units/mL | 10,000 units | US$738 | |
| 10,000 units/mL | 5,000 units | US$410 | |
| 10,000 units/mL | 2,000 units | US$180 | |
| 10,000 units/mL | 2,000 units | US$100 |
Highlights
3’→5’ proofreading exonuclease-High fidelity
Extreme processivity-Generation of large fragments
Isothermal amplification-No need for a thermocycler
Product Source
A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29
Product Usage
Supplied in
10 mM Tris-HCl
100 mM NaCl
0.1 mM EDTA
1 mM DTT
50% glycerol
pH 7.4 @ 25°C
Unit Definition
One unit is defined as the amount of polymerase required to convert 0.5 pmol of dTTP into acid insoluble material in 10 minutes at 30°C
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Phi29 DNA Polymerase
Price range: $100.00 through $738.00
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